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The respective primary antibodies and HRP-conjugated secondary antibodies were diluted 1:2,000 in Can Get Signal Solution 1 (TOYOBO) and 1:5,000 in Can Get Signal Solution 2 (TOYOBO), respectively

Tissue Inhibitor of Metalloproteinase (TIMP) Interactions The balance between MMPs and their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs), determines net proteolytic activity in tissues

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Desired dose in milligrams divided by concentration in milligrams per milliliter, multiplied by 100