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doi: 10.1046/j.1365-2710.1999.00238.x

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Then it was ground in liquid nitrogen, 1 ml of TRIzol (NR0002, Regen, China) was added to the ground sample for lysis, chloroform was added to dissociate the nucleoprotein complex completely, the upper aqueous layer was transferred to isopropanol after centrifugation, and the upper aqueous layer was added to 70% ethanol after centrifugation and washed twice

SSc is a rare autoimmune disease with fibrotic pathology manifesting in the skin and internal organs such as the lungs and heart [25,26,27]