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It has been proved that BaP reduced genome-wide DNA methylation, and activated proto-oncogene by hypomethylation in the promoter region, but silenced tumor suppressor genes by promoter hypermethylation, resulting in cancer initiation and progression

They were then fixed in formalin and processed for histologic and histochemical evaluation and morphometry

doi: 10.1073/pnas.89.15.7189

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The only method currently available to enrich AMPylated proteins in cells, without the need for exogenous labeling, is immunoprecipitation with AMPylation-specific antibodies 20