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For both targets, the assay was evaluated over a concentration range of 0.313 to 320 M and was demonstrated to have a quantitative dynamic range spanning nearly three orders of magnitude, with lower limits of quantification being 0.330 M for GSH and 0.370 M for cysteine

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IsoNet, an isotopologue similarity networking strategy based on mass spectrometry-resolved stable-isotope tracing metabolomics, enables ab initio elucidation of unknown metabolites and their reactions, which is applied to the glutathione metabolic reaction network.