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In the two epilepsy models as mentioned above, the decrease of class I HDACs initiates the acetylation of histone H4 protein in rat hippocampal neurons and thereby activates the factors involved in epileptogenesis such as immediate early genes (IEGs) (e.g

The linker ligation and amplification of methylated fragments could add the affinity enrichment methods with array hybridization to enhance resolution and efficiency of detection including the MethylCap kit (Diagenode), and MethylCollector Ultra kit (Active Motif) with distinct fluorescent dye labeling of inputted and enriched fragments [24, 70]

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A significant obstacle to brain cryopreservation by either freezing or vitrification arises from the osmotic effects of cryoprotective agents, but these effects are particularly pronounced when attempting vitrification

In cases of disease progression, corneal cross-linking, intracorneal ring segments or, in advanced stages, deep anterior lamellar or penetrating keratoplasty represent established therapeutic approaches [139]

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