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Emerging evidence indicates that insufficient maternal folate levels during pregnancy may increase the risk of ASD in offspring, whereas folate supplementation has been associated with reduced neurotoxicity, enhanced neurodevelopment, and attenuation of ASD-related symptoms ( 4.4 Trace elements and nutritional supplementation Children with ASD commonly exhibit elevated oxidative stress, characterized by a reduced glutathione-to-oxidized glutathione (GSH/GSSG) ratio and increased levels of malondialdehyde (MDA), a marker of lipid peroxidation (Song et al., 2024)