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Yeast transactivation assay Yeast transactivation and alanine scanning assays were conducted using either Y2HGold yeast strains (TaKaRa Bio USA, San Jose, CA) harboring a pBridge bait vector or diploid yeast strains obtained by mating Y2HGold (bait) with Y187 (prey) strains, the latter carrying the pGADT7 vector (TaKaRa Bio USA, San Jose, CA)

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Together, studies discussed highlight how nanoformulation strategies may enhance the efficacy, bioavailability, and tumor-targeting of repurposed drugs, while simultaneously modulating the tumor microenvironment and antitumor immunity

ALA also downregulated NF-B and Toll-like receptor 4 (TLR-4) expression in peripheral blood mononuclear cells [39]

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This regulatory positioning aligns it with research into adaptive resilience mechanisms within the central nervous system