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In addition to decreasing oxidative stress in the brain, glutathione improves mental clarity and memory, as well as helps prevent the formation of amyloid plaques.Intracellular GSH levels are inversely related to cell viability under oxidative stress in cultured neurons

Protein-lipid overlay assay After 24 h of transfection, N2a cells were washed twice with TBS (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10% glycerol) and cell extracts were prepared as explained above in TBS supplemented with 0.5% Tx-100 and protease inhibitors

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