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LB plates were made by spotting 50 l of a 10X concentrated overnight culture of E
To address this, we selected the region 2000 bp upstream of VEGFA and IL8 TSSs or mutated the M3992 motif, fused these sequences to a reporter gene expressing firefly luciferase, and then co-transfected 293T cells with them and the STAT6 expression plasmid
Adult worms were let lay eggs and plates transferred to 25 C
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