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The ThT kinetic curve is characterized by a lag time ( t lag , the time in which ThT fluorescence reaches 5% of its maximum), dominated by the formation of oligomeric species not detectable by ThT, followed by an elongation phase, in which pre-fibrillar structures elongate to fiber, normally characterized by a time to half ( t 1/2 , the time in which the ThT fluorescence reaches half of its maximum value) and, finally, a plateau region in which the equilibrium is reached and the ThT fluorescence reaches its maximum value I max (Figure S1 in the Supporting Information)

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Krutler, V., van Gunsteren, W

[DOI] [PMC free article] [PubMed] [Google Scholar] 37.Grabarevic Z, Tisljar M, Artukovic B, Bratulic M, Dzaja P, Seiwerth S, Sikiric P, Peric J, Geres D, Kos J

The history of the U.S

Because B12 is obtained through consuming animal-based products such as meat, milk, cheese, and eggs, vegans are at a higher risk of developing a b12 deficiency