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Volcano plot and GO enrichment revealed that the deletion of Mcp1 resulted in oxidative stress response dysfunction

Also observed in the obese mice were increased mRNA levels of Xbp-1, Grp78 and CHOP, as these are indicators of endoplasmic reticulum stress, and are believed to be activated by obesity
The mixture was then meticulously filtered through 11 m of filter paper (Whatman, England), allowing the mushroom residue to undergo two additional extraction rounds, with the supernatant being collected each time

Though, a signaling function for ROS in activating the ROS scavenging mechanism, which might award defence or resistance to stress, has recently been discovered
