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High performance liquid chromatography For measurements of HCY, cysteine (CYS), GSH, SAM and S-adenosyl-L-homocysteine (SAH), cell precipitates from aliquots A and B were diluted in Milli-q H 2 O 1:2 and the cells disrupted by three freeze/thaw cycles (20 to -20C)

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Behavioral assessment: cerebellar ataxia scoring A protocol for the rapid and sensitive quantification of disease in rodent models of cerebellar ataxia (63) is described in Table 1