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The luminescence of samples and of the GSH standard curve was detected using a Spark Multimode microplate reader (Tecan)

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Simultaneously, the microbiota deconjugates primary bile acids via bile salt hydrolase (BSH) and converts them to secondary bile acids through 7-dehydroxylase activity, modifications that significantly affect bile acid signaling functions (39)

Forskolin, a diterpene adenylyl cyclase activator, elevates cAMP and stimulates CREB-driven transcription, promoting mitochondrial electron transport chain recovery, remyelination, and neurotrophic support, while also exerting anti-inflammatory and antioxidant effects [86]

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