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[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Elliott-Sale K.J., Minahan C.L., de Jonge X.A.K.J., Ackerman K.E., Sipil S., Constantini N.W., Lebrun C.M., Hackney A.C

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Lysate preparation, SDSPAGE and western blot analysis After the treatments, the cardiomyocytes from neonatal and adult mice were collected and lysed by radio-immunoprecipitation assay (RIPA) buffer containing inhibitors of proteases and phosphatases at 4 C for 30 min on the tube rotator

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