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Acylcarnitines were measured using multiple reaction monitoring (MRM) for free carnitine (C 0 , m/z 218 m/z 103) and acetylcarnitine (C 2 , m/z 260 m/z 85) and precursor scan for precursor ions (Q1) of acylcarnitines (C 3 to C 18 , scan range m/z 270 to 502) that generated a product ion (Q3) at m/z 85

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A further feature of interest is that the last three enzymes in the cycle with a specificity for long-chain fatty acids constitute a trifunctional enzyme complex on the inner mitochondrial membrane

doi:10.1186/s12970-020-00377-2

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