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2.7 Aortic ring assay The mouse aortic ring assay was conducted according to a previously described method ( 2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

[PubMed] [Google Scholar] 48.Rizos I

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1,2 The number of patients with diabetes globally is expected to reach 783 million by 2045

Genetically, ASD can be linked to identifiable genetic syndromes, such as Fragile X, Tuberous Sclerosis, Neurofibromatosis I, ADNP syndrome, and copy number variations (Lubbers et al., 2024