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Neuroinflammation is a common underlying factor in many neurodegenerative diseases and cognitive disorders

After incubation at 37 C in a humidified atmosphere of 5% CO 2 /95% air for 1, 2 and 3 days, cells were collected for analysis of the expression of growth hormone receptors by RT PCR and Western blotting

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doi: 10.1073/pnas.92.20.9363

After passing through the 70 m cell strainer (BD Falcon, Bedford, MA, United States), the cell suspension was centrifuged at 300 g for 5 min