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Store in a cool, dry place, protected from direct sunlight

Then, cells were treated according to instructions in a dark environment for 3060 min

Researchers generally refer to oxidative stress when one or more parameters reflecting the balance of free radical processes are disturbed, leading to an increase in the steady-state level of ROS that affects many vital processes

Quantitative determinations for manganese and calcium were obtained by the external standard calibration with four standards (0, 1, 5, 10 g L 1 ) prepared daily in the same buffer used for sample preparation, at the same dilution and HNO 3 concentration