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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mM butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, Valencia, CA, USA) and sonicated for 30 sec in an ice bath (Ultrasonic bath, Branson, MO, USA)

Apatinib and s-1 (tegafur + gimeracil + oteracil potassium) have been approved by the National Medical Products Administration (NMPA) of China for the treatment of GC, and patients can afford these treatments (131)

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Once reconstituted, use within 30 days and keep refrigerated

Many of these new substances appear so fast and with little efficacy support in the literature making it difficult for anti-doping authorities to properly evaluate and consider if they are a problem from the standpoint of performance enhancement