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Pharmacologic interventions for painful diabetic neuropathy: An umbrella systematic review and comparative effectiveness network meta-analysis

doi: 10.1038/s41598-022-12817-x 43

Yasemin BM, Hamdi O, Nazmi O

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They were then incubated with oxygenated isolation buffer [5 mM Na 2 EDTA, 10 mM HEPES (pH 7.4), 0.5 mM DTT, 0.25% BSA, 2.5 mM D-glucose, 2.5 mM L-glutamine, and 0.5 mM dl--hydroxybutyrate sodium salt, oxygenated with an O 2 /CO 2 mixture (19:1, v/v)] at 37 C for 40 min to isolate the upper villus epithelial cells

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