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You attached a his-tag onto your protein of interest while cloning it

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For instance, in the case of advanced/metastatic non-small cell lung cancer (NSCLC) with METex14, GEOMETRY mono-1, a multicenter, open-label, multicohort phase 2 trial, demonstrated that capmatinib was highly effective against tumors in patients with advanced or metastatic NSCLC at stages IIIB and IV, particularly those with METex14 or MET amplification, especially in settings without prior treatment [27]

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M7(R1) requires the assessment of mutagenicity for all potential impurities
