melanotic mass phenotype New alleles of D-2-hydroxyglutarate dehydrogenase enable studies of oncometabolite function in Drosophila melanogaster Frontiers | Mapping phenotypic heterogeneity
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doi: 10.1016/s0006-291x(03)00979-3

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New replication space provided by the transplanted liver combined with suppression of adaptive immunity are plausible determinants providing a selective advantage for high replicator variants

Pellets were resuspended in 40 L lysis buffer (TBS containing 0.7% triton-X, 0.1% tween-20, and 1 Halt Protease and Phosphatase Inhibitor (PPI) Cocktail (ThermoScientific 78440)) and lysed on ice for 20 min
Theyre usually temporary and painless

Hardenberg et al., 2021
