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Cell Signaling Technology, Inc.) was used as an internal nuclear protein loading control, while GAPDH (cat

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[PubMed] [Google Scholar] 30.Okuda K

doi:10.1016/j.ccr.2008.11.012

To determine whether TBB inhibition preferentially triggers apoptosis of senescent cells, Zmpste24 / MEFs treated with TBB were stained for C 12 FDG to label senescent cells, then stained for Annexin V, an early apoptosis marker, and 7-aminoactinomycin D (7-AAD), a membrane impermeable dye that is generally excluded from viable cells as a later apoptosis marker, and finally analyzed with flow cytometry

Smoking in particular has been closely linked to increased risk of anxiety disorders