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Notably, 27-HC-resistant cancer cells acquire ferroptosis resistance, leading to increased tumorigenicity and more metastatic properties, although how these lipids specifically inhibit ferroptosis is unclear 129

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Here we develop a feedingfishing approach coupling membrane editing using optogenetic lipid-modifying enzymes (feeding) with organelle membrane proteomics through proximity labeling (fishing) to elucidate molecular players and pathways involved in the homeostasis of phosphatidic acid (PA), a multifunctional lipid central to glycerolipid metabolism

Methionine: 0.1 to 1 mM in cell culture, 100 to 500 mg per kg in animal models

doi: 10.21769/BioProtoc.3819

(3) compatible with electrospray ionization (ESI) for shotgun lipidomics