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For example, experiments using carotid artery endothelial cells (CAECs) and wild-type mice with partially ligated carotid arteries showed that TMAO significantly induced NLRP3 inflammasome activation and increased caspase-1 activity, IL-1 production, and cell permeability, which contributed to the endothelial injury that initiates AS (Koka et al., 2016

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