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Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections (5 m) were used for haematoxylin and eosin staining and immunohistochemical analyses

These risk factors include: Affected individuals may also develop other autoimmune disorders especially Addison disease, autoimmune thyroid disease (both hyperthyroidism and hypothyroidism), diabetes mellitus, pernicious anaemia and alopecia areata

From this point on, we used Accutase solution to make single-cell suspension for further expansion of NPCs or terminal differentiation into neurons

However, neurotrophins have had little success in clinical trials directed at neurodegenerative diseases due to their poor pharmokinetic profile and large molecular weight that significantly impedes penetration of the BBB [61,62]

Interestingly, the application of DAPT and BMS in these studies seemed to counteract the actions of TB-500, potentially indicating that the mechanisms of action of TB-500 in promoting angiogenesis might be mediated through its interactions with the Notch and NF-B pathways

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