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Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer

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1989 Feb;29(2):90-2

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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Therefore, even classification of the nature of effect into beneficial or adverse may depend on the condition being treated