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Supernatants were applied to a preequilibrated ion-exchange chromatographic column HisTrap HP on an KTA pure chromatography system (Cytiva)

Crit Rev Food Sci Nutr (2021) 61:33755

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: Effects of beta-alanine supplementation on body composition: a GRADE-assessed systematic review and meta-analysis Damoon Ashtary-Larky et al
There is another explanation for the failure of neuropathic pain medications

2.5 Determination of protein concentration The concentration of purified proteins was determined using Bradford reagent (Sigma-Aldrich) according to the manufacturers instructions with bovine serum albumin as a standard
