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Yes, you typically mix the entire vial at once
Here, we propose the misfolding of the mutant ATXN3 protein and the subsequent aggregation process, with eventual deposition of insoluble intracellular aggregates, to be the hub of the pathogenic process, and discuss how protein dyshomeostasis impacts key cellular pathways causing dysfunction and disease (Fig
That's a sprawling subject for another day, but the rule for the diluent itself is clear: keep it refrigerated, not frozen
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