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[44] In the cytosol [edit] Methylcobalamin and 5-methyltetrahydrofolate are needed by methionine synthase in the methionine cycle to transfer a methyl group from 5-methyltetrahydrofolate to homocysteine, thereby generating tetrahydrofolate (THF) and methionine, which is used to make SAMe

I was seriously deficient in vitamin B12 something Id never heard of
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coli DH5 was used to maintain plasmids with broad-range origins of replication
