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Then, the potential induction of SOCE by tBuOOH was evaluated

All the data were integrated by using XDS 61 then merged and scaled using SCALA from the CCP4 suite of programs 62,63

doi: 10.1093/schbul/sbl058

GSH depletion causes ferroptosis in RPE cells To evaluate the mechanism of cell death in GSH-depleted cells, we treated RPE cells with various cell death pathway inhibitors including ferroptosis inhibitors ferrostatin-1 (Fer-1, 8 M), liproxstatin-1 (Lip-1, 600 nM), iron chelator deferoxamine (DFO, 80 M), pan-caspase inhibitor z-VAD-fmk (30 M) to inhibit apoptosis, autophagy inhibitor 3-methyladenine (3-MA, 10 mM), and lysosomal inhibitor bafilomycin A1 (Baf-A1, 75 nM)

The body weights of mice on day 0 and 49 is shown in Fig