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Brain tissues were fixed in 10% formalin (Sigma-Aldrich, HT501128, Massachusetts, USA) for 48 h, dehydrated through a graded ethanol series (Merck, 6417-5, Darmstadt, Germany), cleared in xylene (VWR Chemicals, 133020-7, Mississauga, Canada), and embedded in paraffin wax (Leica Biosystems, 3801320, Illinois, USA)

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