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Structure-guided discovery of the novel covalent allosteric site and covalent inhibitors of fructose-1,6-bisphosphate aldolase to overcome the azole resistance of candidiasis
Samples were post-fixed in osmium tetroxide for 2 h in the dark, dehydrated in graded acetone, and embedded in epoxy resin
Elution conditions for other affinity tags are also generally well tolerated by proteins, so this really isnt a reason to try one tag or another first
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