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Cells were stained overnight at 4C with the following primary antibodies diluted in 1% BSA in PBS: FOXO4 (Cell Signaling Technology Cat# 9472, RRID:AB_10831833, diluted 1:500), CDKN1A (Cell Signaling Technology Cat# 2946, RRID:AB_2260325, diluted 1:500), CDKN2A (Abcam Cat# ab108349, RRID:AB_10858268, diluted 1:500), TP53 (Cell Signaling Technology Cat# 9282, RRID:AB_331476, diluted 1:500), and TP53BP1 (BD Biosciences Cat# 612523, RRID:AB_399824, diluted 1:500)

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Air pollutants may exert their testosterone-lowering effect by triggering oxidative stress and inflammation, as well as interfering with normal testicular cell function

PubMed Rockenstein, E., Torrance, M., Mante, M., Adame, A., Paulino, A., Rose, J.B., Crews, L., Moessler, H., & Masliah, E

I started using the Lipo 10 shots in conjunction with the HCG and liked the results so much that I continue to take them for health and vitality, months later