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1,21 It should be avoided if secondary bacterial infection is present

In brief, cells were washed twice with phosphate-buffered saline and resuspended in prewarmed (37 C) Seahorse basal medium (Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 5 mM glucose (pH 7.4)

Western blots The total amounts of hippocampal proteins (20 g) from three mice of each group were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (cat# IPVH00010, Merck millipore, USA)

In IH-treated SD rats, the level of autophagy activated, inhibiting the p38 mitogen-activated protein kinase (MAPK) signaling pathway could further activate autophagy in hippocampal nerve cells, thus reducing nerve cell injury [60]

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Eine Infusionstherapie sollte in der Regel schmerzfrei sein