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Slices were cultured in Minimum Essential Medium (MEM) with Hanks balanced salts [Thermo Fisher Scientific, #11575032], 1% penicillinstreptomycin and 25% horse serum [Thermo Fisher Scientific, #16050130] for 3 days, with 1 mL of medium added below the membrane insert

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Restoring NAD+ levels in animal models has reversed multiple aging biomarkers
