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10.3109/02844318809097928 [DOI] [PubMed] [Google Scholar] Herchenhan A., Bayer M

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After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Fisher Scientific, 1:5000), the proteins were visualized using chemiluminescence reagents (29050, Engreen, San Jose, CA, USA) with a Gel Doc EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed using the Image Lab software (Bio-Rad Laboratories)

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J Mol Biol 1984;174(3):483-96

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Next, cells were washed with PBS, incubated for a further 6 to 12 h, lysed, and bacterial enumeration was conducted to assess the internalization capabilities

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5.4 Comparative therapeutic efficacy The translation of therapeutic agents from animal models to clinical trials faces significant barriers

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