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Cells were seeded at a density of 1 10 6 cells/mL and were cultured for 24 hours (n = 11) or 72 hours (n = 19) in RPMI-1640 medium supplemented with 10% human serum (Sigma-Aldrich) and penicillin-streptomycin (100 U/g/mL)

Shaking creates foam and can mechanically destroy the peptide molecules, rendering your sample useless

Crit Care Med 28:18031807 Deo AK, Bandiera SM (2008) Biotransformation of lithocholic acid by rat hepatic microsomes: metabolite analysis by liquid chromatography/mass spectrometry

Neuroinflammation 11:101262

Then, the EEPT value is close to zero, and therefore, the EEPT/NEPT coefficient tends towards zero, which brings the solution of the equation closer to (or equal to) the value of 1

Published 2018 Sep 26