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Supernatants from cell cultures of established tumor cells were collected to assess the specific binding of VEGFR2-Fc (Supplementary Fig

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As altitude increases, the atmospheric oxygen pressure decreases exponentially, leading to hypoxia

Methods Cell culture Human lung adenocarcinoma cell lines (A549, H322, H1299, H1792, H1975, H2009, PC9) were previously obtained 87 from the Harmon Cancer Center Collection (University of Texas-Southwestern Medical Center) or commercially from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium (Fisher Scientific, 11-875-119) supplemented with 5% FBS (Sigma-Aldrich, F0926) in the absence of antibiotics

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