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The beads were washed five times with lysis buffer, and the bound proteins were eluted with Laemmli buffer

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These include sustaining proliferative signaling, evading growth suppressors, resisting cell death, enabling replicative mortality, inducing or accessing vasculature, activating invasion and metastasis, deregulating cellular metabolism, avoiding immune destruction, genome instability and mutation, tumor-promoting inflammation, unlocking phenotypic plasticity, nonmutational epigenetic reprogramming, polymorphic microbiomes, and senescent cells [2]