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Thus, dysregulated CS activation, such as the alternative CS signaling pathway in women who suffered RPL previously, predisposes them to develop preeclampsia during a successful pregnancy due to their altered CS signaling pathway, even in the presence of healthy placental development

STATISTICAL ANALYSIS Statistical analysis of the data was carried out using GraphPad Prism 6

In the experiment, Saccharomyces cerevisiae fungus with an expression plasmid containing the cDNA of the human estrogen receptor and a reporter plasmid having the -galactosidase gene were utilized [7]

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