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The hypothalamic-pituitary-gonadal axis often experiences significant suppression during steroid cycles, and restoration of normal function can take months without intervention

In muscle tissue, NAD+ availability is critical for the function of sirtuins, especially SIRT1 and SIRT3 , which regulate mitochondrial biogenesis, oxidative metabolism, and cellular stress responses

All methods involving human cells and tissue were carried out in accordance with relevant guidelines and regulations of the Ethics Committee of Capital Medical University

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