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The obtained tissue explants were cultured in DMEM/F-12 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 2% penicillin/streptomycin (Sigma, Germany) and incubated at 37 C and 5% CO 2 until the outgrowing cell confluence reached 80%

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Frontiers in Molecular Neuroscience 11: 10
