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Insulin resistance was assessed by ITTs in which mice were injected with 1 (1.5 for high-fat diet-fed mice) U/kg body weight Actrapid (Novo Nordisk, Denmark)

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The show kicked off with two tracks from the earlier album, Caught Somewhere in Time and Stranger in a Strange Land. The second marked the first appearance of Maidens skeletal mascot Eddie, a 12-foot-tall version who briefly strode on stage sporting a trench coat and wide-brimmed hat