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The beads were washed three times with washing buffer (50 mM Tris pH 8.0, 150 mM NaCl, 0.1% Triton, and 5 mM EDTA), boiled in 50 l 2 SDS loading buffer, and then subjected to western blot analysis
This ensures efficient transport of lipid nutrients to oocytes, promotes yolk formation and lipid deposition, and ultimately improves egg production rates
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Cell 62 , 385396 (2016)
Lpez-Miranda, J
A local anesthetic like lidocaine or bupivacaine
