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Five different sources of contrast were used to image the hair follicle: the autofluorescence of the cells NADH, the emission of second harmonic generation (SHG) by the collagen, the emission of third harmonic generation (THG) mainly by cell membranes, the Hoechst signal from the labelled nuclei, and the transmission of brightfield illumination

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It is needed for neurotransmitter reuptake, endocytosis of SV, and presynaptic vesicles, let alone maintain the concentration gradients of ions across the membrane