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FIGURE 1 The MAO enzyme has two isoforms, MAO-A and MAO-B, which share approximately 70% sequence homology but differ in substrate specificity, inhibitor selectivity, and tissue distribution ( Nevertheless, a new generation of selective MAO-B-inhibiting agents has demonstrated efficacy in alleviating PD symptoms, and these inhibitors do not cause any kind of adverse effects ( 2 O 2 ), culminating in oxidative damage and apoptotic signaling events ( 50 value of 450 nM and a selectivity index exceeding 700 ( 2A receptor antagonist, exhibits a dual mechanism of action by also acting as an MAO-B inhibitor, making it a promising candidate for treating PD

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In each method solvents A and B comprising of water-acetonitrile-formic acid (90:10:0.1, v/v/v) and methanol-acetonitrile-formic acid (90:10:0.1, v/v/v), respectively were used