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doi: 10.1001/jama.2017.7105

with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)

Constitutive MECP2 knockout (KO) mice, designed to study MECP2 loss, exhibit severe neurological symptoms such as uncoordinated gait, hindlimb clasping, and irregular breathing after an initial period of normal development (111, 112)

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Thomas, D