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Second, there was no control group, so we cant rule out the possibility that the patients would have improved over time anyway
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The samples were then incubated with the equilibration buffer provided in the TUNEL assay kit at room temperature for 10 min, followed by incubation with the reaction mixture containing terminal deoxynucleotidyl transferase and labeled dUTP

10.1016/S0892-0362(02)00218-0

Cell Metab 31 , 267283.e12 (2020)

[75] The loss of pericyte cells will progressively change the structure of retinal microvessels, disrupting the BRB
