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Thus, aldose reductase activity consumes NADPH (Figure 4), a critical cofactor necessary for the regeneration of the primary lens antioxidant, glutathione (GSH), from its oxidized form (GSSG)

Additionally, mercury orange staining and FACS analysis revealed that Sag caused a depletion of intracellular glutathione (GSH) specifically in Daudi, Raji, and Jurkat cells, but not in Hut-78 or normal BT cells

doi: 10.1016/j.fhfh.2022.100110 214

Tate SS, Meister A

After that, the mixture was fractionally distilled and collected into three separate fractions under reduced pressure as described by Rabbani and Thornalley 60
